Structure and Bioactivities of a Carboxymethyl Polysacchairde from Pachyman
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Abstract:
A carboxymethyl polysaccharide from Pachyman was isolated and purified using DEAE-52 cellulose column, Sephadex-G200 column and Sephadex-G150 column in the work. The structure of this polysacchairde was identified and its anti-proliferation activity and anti-inflammation activity investigated. This polysaccharide is a (1→3)-β-D-glucan with an average molar mass of 20.96×104 u, which also has a little (1→2) glucosidic bonds. Its monosaccharide composition only contained D-glucose and the triple helical structure did not exist. This polysacchairde exhibited a strong anti-proliferation effect on HT-29, HepG-2, SGC-7901, MCF-7 and A549 cells, with IC50 values of 140.5 μg/mL, 264.3 μg/mL, 102.5 μg/mL, 256.4 μg/mL and 313.2 μg/mL, respectively. In addition, this polysaccharide exhibited an inhibitory effect on LPS-induced RAW264.7 cells, but could activate RAW264.7 cells in the absence of LPS. The findings would be of great importance for the development of Pachyman polysaccharide-based health foods and drugs.
