Protective Effects of Active Components Extracted from Chaenomeles Speciosa on Non-alcoholic Fatty Liver Disease
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Abstract:
The protective effects of active components extracted from Chaenomeles Speciosa (EACCS) on non-alcoholic fatty liver disease (NAFLD) in mice were investigated. Forty male KM mice were equally randomized into four groups, namely, normal group, model group, low-dose EACCS group (100 mg/kg body weight/day), and high-dose EACCS group (300 mg/kg body weight/day). The normal group was fed with a normal diet, the model group was given a high-fat–high-fructose diet (HFFD), and the low-dose and high-dose EACCS groups were given HFFD followed by EACCS at low- and high-dose, respectively. Body weights were monitored. The mice were sacrificed 1 month later and serum-based biomarkers and morphology of liver tissue were examined. Real-time (RT)-PCR and confocal microscopy techniques were used to detect the expression of lipid metabolism-related genes. Compared with the normal group, the serum alanine aminotransferase (ALT) and total cholesterol (TC) of the model group increased, indicating significant steatosis; The expression levels of hepatocyte growth factor (HGF), vascular endothelial growth factor-A (VEGF-A) and c-Met also decreased in the model group, whilst the expression level of miR-199a-5p increased. Compared with the model group, the hepatic lipid accumulation of EACCS groups was obviously suppressed with significantly decreased serum ALT and TC levels and the expression levels of hepatic HGF, VEGFa and c-Met mRNA increased; and the expression levels of miR-199a-5p increased. Therefore, EACCS has protective effects on NAFLD mice induced by HFFD, and its mechanism may be related to the activation of miR-199a-5p-HGF/c-Met signaling pathway in the liver tissues.
