Protection of Viscera in Rat with Alcohol-induced Liver Injures by Glycyrrhizic Acid: Influence of the ratio of 18α-, 18β-Glycyrrhizic Acid
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Abstract:
The objective of this paper was to investigate the effect of different proportions of 18α- and 18β- glycyrrhizic acid (Gly) on the morphology of organs in rats with alcoholic liver injury. Healthy male rats were randomly divided into ten groups, including normal control group, model group, positive control group (silymarin), 7 drug groups (18α-Gly and 18β-Gly group 10.83 mg/kg at ratios of 10:0, 8:2, 6:4, 5:5, 4:6, 2:8, 0:10). At the fourth week, important organs were collected and weighted, including liver, kidney and spleen, which were then stained by routine oil red O and hematoxylin-eosin. The histology of organs was observed by optical microscope. The results showed that 18α-Gly and 18β-Gly at the ratios of 2:8 significantly decreased organs indexes of respiratory and digestive system, obviously reduced muscle index and increased brain index, and meanwhile decreased cardiac index and spleen index. Additionally, 18α-Gly and 18β-Gly at the ratios of 4:6 and 2:8 reduced hepatocytes steatosis and lipid accumulation. 18α-Gly and 18β-Gly at the radio of 4:6 or less could decrease inflammation of the kidney, and meanwhile, alleviated injury to spleen demonstrated by the clear boundary of red and white pulp of spleen. Collectively, the optimal compatibility proportion of 18α-Gly and 18β-Gly on protecting organ injury induced by alcohol was 4:6 and 2:8.
