Determination of Total Flavonoids in Celery by XC-72/GCE
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Abstract:
A glassy carbon electrode modified with XC-72 conductive carbon black was prepared by a simple drop coating method to study the electrochemical behavior and determination of rutin and total flavonoids in celery. Cyclic voltammetry (CV) was used to optimize the electrochemical reaction conditions, including accumulation time, scan rate and pH of the buffer solution. Under the optimal conditions, the standard curve of rutin was established using differential pulse voltammetry (DPV) method for the determination of total flavonoids in celery. The results showed that the XC-72/GCE electrode had good electrocatalytic activity for the oxidation of rutin, and the electrode reaction was a process of one-electron and one-proton transfer with a electron transfer coefficient (α) 0.51. The DPV oxidation peak current (Ip) of rutin had a good linear relationship with its concentration in the range of 4.1×10-8 ~ 1.3×10-5 mol/L with a correlation coefficient of 0.9985 and a detection limit of 4.1×10-14 mol/L (S/N=3). The modified electrode had the advantages of simple preparation, fast response, repeatability, good stability and strong anti-interference capability, which could be used as a high sensitive electrochemical sensor for the determination of total flavonoids in celery. In addition, this research can also provide reference for the determination of total flavonoids in other vegetables.
