Simultaneous Determination of γ-antisterility vitamin, β-sitosterol, Squalene in Camellia Oil by HPLC
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Abstract:
A high performance liquid chromatography (HPLC) method was developed for the simultaneous determination of γ-antisterility vitamin, β-sitosterol, squalene in camellia oil. The camellia oil samples were saponified, extracted and concentrated to remove the saponifiable substance in camellia oil, and the unsaponified material component was enriched. The content of the saponification products were determined by HPLC and analyzed by external standard method. Hypersil ODS C18 (250 mm × 4.6 mm, 5 μm) chromatography column was used for the determination under the conditions of mobile phase methanol (volume ratio 100%) and detection wavelength 205 nm. The column temperature was 30 oC and the flow rate was 0.7 mL/min. The results showed that correlation coefficients were all close to 1 for γ-antisterility vitamin, β-sitosterol and squalene in the range of 0.01~0.40 μg (R2=0.9999), 0.44~10.94 μg (R2=1) and 0.01~0.55 μg (R2=0.9990), respectively. reThe average recovery was 97.1322% (RSD=1.28%), 100.2346% (RSD=1.54%), 98.0859% (RSD=1.58%), and the relative standard deviations were all less than 3%. The proposed method had the advantages of simple pretreament, easy operation, high sensitivity, good accuracy and reproducibility, and could be used for the determination of γ-antisterility vitamin, β-sitosterol, squalene in camellia oil and the evaluation for the quality of camellia oil.
