Rapid Detection of Alflatoxin B1 by Magnetic Bead Immunoassay Based on Copper Chromogenic Reaction
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Abstract:
A method of colorimetric immunoassay based on functionalization of copper oxide nanoparticles was established for the rapid and sensitive detection of Alflatoxin B1 (AFB1), a tumor biomarker. The method was established based on that the carboxyl covalent bonding magnetic bead surface antibody was highly bound to aflatoxin B1, and then was specifically recognized by the electrostatic adsorption on the surface of the nano copper oxide, and the quantitative bound copper oxide marker could release high concentration of copper ions by acid solution, and then the copper ion chromogenic agent of the hydrazone could generate red complexes. The absorbance of the red solution was determined by the ultraviolet visible absorption spectrometry, or directly through the visual colorimetric method, which could easily realize the signal transduction of immune analysis so as to realize rapid detection of aflatoxin B1. The results showed that the method had a high detection sensitivity and a good correlation in the range of 0.01 to 100 ng/mL when the incubation time was 15 min and the oncentration of chromogenic agent was 10 μmol/L. In addition, this method has been successfully applied to the analysis of alflatoxin B1 in rice samples, and the results were consistent with those in GB 5009.22.
