Cloning and Analysis of UDP-Glucose:Flavonoid 5-O-Glucosyl Transferase(5GT) Allele in Vitis amurensis
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Abstract:
A large number of diglucosidic anthocyanins in Vitis amurensis seriously affect the quality of wines, and 5GT is the key enzyme for the synthesis of diglycoside anthocyanins. Therefore, studying the differences of 5GT alleles among different grape varieties lay the foundation for inhibiting the synthesis of diglucosidic anthocyanins, which is of great significance for improving the quality of wines made from Vitis amurensis grapes. The 5GT alleles in ‘Cabernet Sauvignon’, ‘Zuoshanyi’, ‘Hasang’ and ‘Zuohongyi’ were cloned and analyzed in this study by sequence analysis and bioinformatics analysis, and seven 5GT alleles were all on chromosome 9 and encoded 297~464 amino acids. The results of sequence analysis showed that four 5GT alleles might lose the function of 5GT due to the gene mutation, seven5GT alleles had no signal peptide and belongs to the 5GT subfamily of GT1. There were some differences in 5GT alleles among different grape varieties. from which we could speculate that only three alleles could synthesize diglucosidic anthocyanins.
