Isolation and Purification of Polysaccharide from Grifola frondosa and Its in vitro Antitumor Activity
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
The structure and in vitro anti-tumor activity of polysaccharides extracted from Grifola frondosa at low temperature were investigated in this study. Grifola frondosa polysaccharides were extracted by alcohol precipitation at low temperature (4℃), and the proteins in crude polysaccharides were removed by Sevage method. The small molecule substances such as salts and oligosaccharides were removed by dialysis to obtain the polysaccharide fraction named as GFP. The yield was 2.13%. The main monosaccharides were glucose, galactose and mannose detected by ion chromatography. The results of HPLC analysis showed that GFP was a mixture containing two major macromolecules with molecular weights of 1700.00 ku and 34.12 ku, respectively. The peak areas of the components were 66.57% and 28.23%, respectively. Infrared spectra showed that GFP might be α-pyran polysaccharide. The anti-tumor activity of GFP showed that GFP could significantly inhibit the proliferation of human hepatic hepG2 cells within a certain concentration range; Detection of mitochondrial membrane potential by JC-1 staining revealed that GFP caused a decrease in mitochondrial membrane potential, indicating that GFP-induced apoptosis was accompanied by changes in membrane potential. Morphological observation showed that the GFP-treated cells showed the typical morphological changes of apoptosis, and the cells contracted to form micronuclei. All of the above showed that GFP induced HepG2 cell apoptosis.
