Active Ingredients and Active Function of Extracts from Heracleum Moellendorffii Hance by Different Solvents
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Abstract:
In order to study the active function of extracts from Heracleum Moellendorffii Hance by different solvents, the Heracleum Moellendorffii Hance was used as the raw material in this study. The active ingredients and antioxidant effects of different solvent extracts were compared. The effects of different extracts on the α-glucosidase and α-amylase were studied, and The effects of different solvent extracts on the HepG2 and panc-1 cells under the optimal conditions were also investigated. The results showed that with the increase of the concentration, the four extracts’ inhibitory rate of α-glucosidase and α-amylase also increased. The IC50 values of the two kinds of enzyme inhibitory rates were obtained, and the IC50 values of α-glucosidase and α-amylase in alkali extraction were the minimal value, which were 0.47 mg/mL and 2.17 mg/mL, respectively. The highest content of polysaccharides in the four solvent extracts was alkali extraction, the content was 58.09 mg/g; The highest content of total phenols was ethanol extract, the content was 11.48 mg/g; The highest content of flavonoids was ethanol extract, the content was 3.96 mg/g. The alcohol extract of the four extracts had the highest antioxidant activity, and the acid extract had the lowest antioxidant activity. All the four extracts could promote the proliferation of HepG2 and panc-1 cells at low concentrations. With the increase of concentration, the survival rates of HepG2 and panc-1 cells decreased in different degrees. Extracts had inhibitory effect on cells under high concentration.
