Molecular Mechanism of Kinsenoside in Promoting Glucose Uptake in Skeletal Muscle Cells
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Abstract:
In this study, rat skeletal muscle myoblasts were induced to differentiate into mature myotubes. The effect of kinsenoside on the Akt and AMPK activation was examined by western blot and glucose uptake was measured by fluorescence-labeled 2-deoxyglucose in both insulin-sensitive and insulin-resistant myotubes. The results showed that in normal cells, treatment with 10 nM kinsenoside for 24 h increased the activity of Akt (34.53%, p < 0.05) and AMPK (149.92%, p < 0.05), and simultaneously promoted cellular uptake of glucose effectively (43.35%, p < 0.05). In insulin resistant cells, the impaired insulin-stimulated Akt activation was ameliorated (79.05%, p < 0.05) by kinsenoside treatment. In the case of insulin sensitive and insulin resistant state, kinsenoside exhibited synergistic effect on the insulin-induced glucose uptake. These results suggested that kinsenoside might overcome insulin resistance via modulating both Akt and AMPK activity, leading to increase glucose uptake in insulin resistant cells.