Components Analysis of the Antidepressant 20(S)-Protopanoxadiol Type Ginsenosides
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Abstract:
The 20(S)-protopanoxadiol type (PPD) ginsenosides of the antidepressant total saponins extracted from the leaves of Panax Notoginseng (TSLPN) were investigated in this study, and the ginsenoside composition of TSLPN were analyzed by reference substances and literature. The content of 5 representative PPD ginsenosides including ginsenoside Rb1, Rc, Rb2,Rb3 and notoginsenoside Fc was determined simultaneously by the high performance liquid chromatography (HPLC) with the chromatographic condition as follows: mobile phrase, methanol-0.2% phosphoric acid solution (70:30), detection wavelength, 203 nm, colunm temperature, 30 oC. Results showed that 15 components including Rb1, Rc, Fc, Rb2, and Rb3 were identified, of which 13 components were PPD ginsenosides. Rb1, Rc, Fc, Rb2, and Rb3 were well seperated by HPLC, and the resolutions were above 1.5. In addition, the average recovery rates were 98.92±2.67% (RSD=2.73%), 99.51±1.33% (RSD=1.34%), 100.09±1.45% (RSD=1.45%), 100.53±2.24 (RSD=2.22%), 100.12±1.74% (RSD=1.74%), respectively. Consequently, TSLPN was mainly composed of the PPD ginsenosides, and the total content of Rb1, Rc, Fc, Rb2, and Rb3 was as high as 38.67±0.18%.
