Analysis of Bacterial Community Structure in Medium Temperature Daqu and High Temperature Daqu of Luzhou-flavor Liqu by High-throughput Sequencing
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Abstract:
The medium temperature Daqu and high temperature Daqu of Luzhou-flavor Liqu were analyzed by High-throughput sequencing through Illumin Miseq 2×300 bp sequencing platform, which revealed that 7 phyla and 19 genera were obtained from medium temperature Daqu and 15 phyla and 26 genera were obtained from high temperature Daqu at the threshold of abundance > 0.01%. The first group detected from the medium temperature Daqu was Pediococcus, Pantoea and Melghirimyces. The strains detected in the high temperature Daqu were Streptophyta, Brevibacterium, Thermus, Saccharopolyspora, Citrobacter, Streptococcus, Brevundimonas, and Allobacillus. In addition, the study also found that Bacillus and Thermoactinomyces in the high temperature Daqu were significantly increased compared to the bacteria in medium temperature Daqu , while Kroppenstedtia and Lactobacillus were significantly decreased, probably due to the unique production Daqu technology of Zhanggong. In this study, we established a high-throughput sequencing technique to analyze the bacterial community structure of Luzhou-flavor liquor, which provided theoretical basis for establishing microorganism information database of Luzhou-flavor liquor, optimizing Daqu fermentation technology and improving quality.
