Development on Quinolone Immunochromatographic Test Strips Labelled with Blue Latex Microspheres
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Abstract:
Quinolones were widely used in livestock breeding industry as the broad-spectrum antimicrobial drugs. However, veterinary drugs would remain in animal-derived foods if the use of quinolones was not standardized, causing the potential risks to consumers’ safety.. Therefore， it was inevitable to establish a rapid and simple detection method for quinolones. In this study, blue latex microspheres were used as a marker material to mark norfloxacin monoclonal, and the established method of blue latex microsphere-labeled quinolone immunochromatographic test strips was optimized to provide theoretical support for rapid detection of veterinary drug residues in foods. The detection limit of this method was 1 μg/L under optimized the conditions, and samples of pork, shrimp and chicken that might have veterinary drug residues were selected for the recovery test, the detection limits of the samples were all 10 μg/kg. The established method could detect 6 quinolone residues, including ciprofloxacin, enrofloxacin, fleroxacin, marbofloxacin, enoxacin and norfloxacin, and the test trips have good stability with an effective period of at least three months.
