Optimization of Extraction Process of Polyphenols from Kumquat by Response Surface Methodology
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Abstract:
The optimum extraction process of polyphenols from Kumquat was investigated. Based on singe factor experiments (extract time, extract concentration, ratio of liquid to material, etc.), the extraction conditions of free polyphenols and bound polyphenols in kumquat were optimized by response surface methodology using polyphenol content as response value. The results showed that the established regression models of the free polyphenols and bound polyphenols of kumquat were significant and it could be used to predict the yield of free polyphenols and bound polyphenols. The optimum extraction conditions of free polyphenols were as follows: the acetone concentration of 77%, the liquid to material ratio of 33 mL/g; the extraction time of 48 min. The average free polyphenol content under this condition was 11.03 mg/g DW. The optimum extraction conditions of bound polyphenols were as follows: the NaOH concentration of 6.5 mol/L; the liquid to material ratio of 10 mL/g; the extraction time was 18 h. Under these optimized conditions, the yield of bound polyphenols reached 1.79 mg/g DW. The content of free polyphenols and bound polyphenols under optimal conditions were well matched with the model predicted yield, indicating that the optimized extraction process of free and bound polyphenols from kumquat was reasonable.
