Abstract:This paper investigated the optimal parameters for high-density inoculation of Coccomyxa subellipsoidea C-169 under different nutritional conditions in yeast fermentation wastewater. The effects of inoculation density and wastewater concentration on the growth of C-169 and the treatment of yeast wastewater were systematically compared. Results showed that C-169 could use glucose and sucrose for heterotrophic and mixotrophic cultivation. Under cultivation conditions, the growth rate of C-169 was the fastest and the optimal glucose concentration was 20 g/L. The effects of culture conditions (inoculation density and initial wastewater concentration) on the growth of C-169 and the treatment of yeast wastewater were studied by single-factor experiments , and the optimal culture conditions were as follows: the initial inoculum density was not less than 1 g/L, and the dilution rate of decolorization wastewater was 2 times. Under this condition, the biomass yield of C-169 was 0.23 g/(L·d), and the removal rate of COD (chemical ocygen demand), total nitrogen and total phosphorus in decolorization wastewater were 49.54%, 70.39%, 98.09%, respectively. Consequently, this study illustrated that C-169 had great potential for the purification of yeast wastewater under algal-bacterial coexistence conditions.
