Abstract:Cinnamic alcohol ester were synthesized with cinnamic alcohol in solvent-free system using Candida antarctica lipase B-displaying Pichia pastoris whole-cells as catalyst and fatty acids as acyl donors. The selectivity of Pp-CALB for acyl donors was investigated, and the reaction temperatures, agitation speeds, enzyme dosage, molar ratio of substrates, initial water activity of Pp-CALB and reaction system were optimized to establish enzymatic synthesis process of cinnamyl alcohol ester in solvent-free system . The optimal reaction conditions were as follows: reaction temperature was 50 ℃, molar ratio of acid to alcohol was 2:1, agitation speed was 120 r/min, initial water activity of Pp-CALB was 0.53, and enzyme loading was 0.05 g. The reaction conversion reached 81.34% under the optimal conditions sysnthsized for 3 h, which could still reach more than 70.00% after 10 cycles of continuous reaction with Pp-CALB, indicating the good operational stability.
