Determination of Abamectin Residues in Cabbage and Orange by Ultra Performance Liquid Chromatography
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Abstract:
A determination method for abamectin residues in cabbage and orange was established in this study by using ultra high performance liquid chromatography- ultraviolet (UPLC-UV). Samples were extracted with acetonitrile, and mixed with ethylenediamine-N-propylsilane (PSA) and C18 matrix dispersion scavenger for purification by using a ACQUITY UPLC BEH C18 column (2.1×100 mm, 1.7 μm). The column temperature was set as 35 ℃, the mobile phase was composed of acetonitrile-water (70:30, V/V) with a flow rate of 0.25 mL/min, and the ultraviolet detection wavelength was set as 245 nm. Abamectin had different matrix effects on cabbage and orange samples and therefore, external standard of the blank matrix-matched calibration curves was used for quantification. The results showed that abamectin had good linear relationship in the range of 0.25~10 mg/L, and the correlation coefficients were all more than 0.9999. In the range of 0.05~0.5 mg/kg, the average addition recovery was 83.0%~100.2%, and the relative standard deviation (RSD) was 1.17%~1.30%. The limits of detection (LOD) of abamectin in cabbage and orange (S/N=3) were 0.0109 mg/kg and 0.0160 mg/kg, respectively. The proposed method was simple, accurate and sensitive, which could be used for the analysis of abamectin in cabbage and orange.
