Simultaneous Determination of Carbamazepine and Its Metabolites in Animal Matrix Using Ultra Performance Liquid Chromatography Tandem Mass Spectrometry
Carbamazepine is a drug for the treatment of psychiatric illnesses, such as epilepsy, trigeminal neuralgia and so on. However, it also can stimulate the release of antidiuretic hormone, causing side effect of water-sodium retention. Water-sodium retention can lead to tissue edema and weight disorders, which provides the possibility of water retention and antidiuretic by illegal water injection in the breeding process. Therefore, it is of great significance to detect carbamazepine in animal tissues for cracking down on illegal water injection. A multiresidue method based on ultra performance liquid chromatography tandem mass spectrometry was developed for the determination of carbamazepine and its metabolites in animal matrix. In this method, samples were extracted by acidified acetonitrile, and purified by Oasis PRiME HLB SPE column, and then carbamazepine and its metabolites were analyzed by Acquity UPLC BEH C18 column (100 mm×2.1 mm, 1.7 μm) using methanol and 0.1% formic acid as the mobile phases. The signals were acquired through the MRM mode. All analytes could be well-separated by a gradient program during 8 minutes. The calibration curves of carbamazepine and its metabolites had a good linear in a concentration range of 1.0~50.0 μg/L with the correlation coefficient more than 0.999, and the limits of quantitation were all less than 1.0 μg/kg in animal matrix. The recoveries of 1.0, 10, 20 μg/kg fortified samples ranged from 72.3% to 106.8%, with RSDs of 1.43% to 10.3%. The new method provides a certain reference for the prevention of illicit drug addition and water injection in animal products.
[1] Alrashood S T. Chapter three-Carbamazepine [J]. Profiles of Drug Substances, Excipients and Related Methodology, 2016, 41: 133-321
[2] 喻东山.卡马西平不良反应和药物相互作用的研究进展[J].医药导报,2010,29(10):1281-1284
YU Dong-shan. Advances in the study of carbamazepine adverse reactions and drug interactions [J]. Herald of Medicine, 2010, 29(10): 1281-1284
[3] Roberto M, Fiorenzo A, Giovanna C, et al. Simultaneous high-performance liquid chromatography determination of carbamazepine and five of its metabolites in plasma of epileptic patients [J]. Journal of Chromatography B: Biomedical Sciences and Applications, 2001, 762(2): 109-
116
[4] Van R G F, Badenhorst D, Swart K J, et al Determination of carbamazepine and carbamazepine 10,11-epoxi- de in human plasma by tandem liquid chromatography-mass spectrometry with electrospray ionization [J]. Journal of Chromatography B, 2002, 769(1): 1-7
[5] ZHU Yong-xin, CHANG Hua, Wulster-Radcliffe M, et al. Liquid chromatography/tandem mass spectrometry for the determination of carbamazepine and its main metabolite in rat plasma utilizing an automated blood sampling system [J]. Journal of Pharmaceutical and Biomedical Analysis, 2005, 38(1): 119-125
[6] Lopes B R, Barreiro J C, Baraldi P T, et al. Quantification of carbamazepine and its active metabolite by direct injection of human milk serum using liquid chromatography tandem ion trap mass spectrometry [J]. Journal of Chromatography B, 2012, 889: 17-23
[7] Kanakapura B, Sameer A M. Utilization of bromination reactions for the determination of carbamazepine using bromate-bromide mixture as a green brominating agent [J]. Arabian Journal of Chemistry, 2014, 7(3): 297- 305
[8] 陈世文,刘玲,姜宁.紫外分光光度法测定癫痫患者的卡马西平血药浓度[J].中国药业,2010,19(9):18-19
CHEN Shi-wen, LIU Ling, JIANG Ning. Determination of Serum carbamazepine concentration by UV-spectrophoto -mentry [J]. China Pharmaceuticals, 2010, 19(9): 18-19
[9] Maryam V, Shiva G, Masoumeh S. Chemometrics enhanced HPLC-DAD performance for rapid quantification of carbamazepine and phenobarbital in human serum samples [J]. Talanta, 2014, 119(5): 17-23
[10] 阙万才,丘宏强,程昱,等.固相萃取_高效液相色谱法测定人血浆中奥卡西平及其代谢产物10-羟基卡马西平的浓度[J].中国临床药理学杂志,2015,31(22):2254-2256
QUE Wan-cai, QIU Hong-qiang, CHENG Yu, et al. Determination of oxcarbazepine and its metabolite 10,11-dihydro-10-hydroxy carbamazepine in human plasma by solid phase extraction and HPLC [J]. Chin. J Clin. Pharmacol., 2015, 31(22): 2254-2256
[11] WU Shu-chao, XU Wei, Qamar S, et al. Ion chromatography combined with online electrochemical derivatization and fluorescence detection for the determination of carbamazepine in human plasma [J]. Talanta, 2012, 101(22): 541-545
[12] QU Li-hua, FAN Yuan-jie, WANG Wen-jun, et al. Development, validation and clinical application of an online-SPE-LC-HRMS/MS for simultaneous quantification of phenobarbital, phenytoin, carbamazepine, and its active metabolite carbamazepine 10,11-epoxide [J]. Talanta, 2016, 158: 77-88
[13] 戴博,张华峰,宋青,等.高效液相色谱-质谱联用法同时测定苯巴比妥、丙戊酸钠、苯妥英钠和卡马西平的血药浓度[J].中国临床药理学杂志,2011,27(3):213-215
DAI Bo, ZHANG Hua-feng, SONG Qing, et al. Determination the concentrations of phenobarbital, sodium valproate, phenytoin sodium and carbamazepine in human plasma for simultaneous quantification by HPLC-MS [J]. Chin. J Clin. Pharmacol., 2011, 27(3): 213-215
[14] TEO Hui-ling, WONG Ling-kai, LIU Qin-de, et al. Simple and accurate measurement of carbamazepine in surface water by use of porous membrane-protected micro-solid-phase extraction coupled with isotope dilution mass spectrometry [J]. Analytica Chimica Acta, 2016, 912: 49-57
[15] Oberleitner L, Dahmen-Levison U, Garbe L A, et al. Application of fluorescence polarization immunoassay for determination of carbamazepine in wastewater [J]. Journal of Environmental Management, 2017, 193: 92-97
[16] Behbahani M, Najafi F, Bagheri S, et al. Application of surfactant assisted dispersive liquid-liquid microextraction as an efficient sample treatment technique for preconcentration and trace detection of zonisamide and carbamazepine in urine and plasma samples [J]. Journal of Chromatography A, 2013, 1308(14): 25-31
