Simultaneous Determination of Carbamazepine and Its Metabolites in Animal Matrix Using Ultra Performance Liquid Chromatography Tandem Mass Spectrometry
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Abstract:
Carbamazepine is a drug for the treatment of psychiatric illnesses, such as epilepsy, trigeminal neuralgia and so on. However, it also can stimulate the release of antidiuretic hormone, causing side effect of water-sodium retention. Water-sodium retention can lead to tissue edema and weight disorders, which provides the possibility of water retention and antidiuretic by illegal water injection in the breeding process. Therefore, it is of great significance to detect carbamazepine in animal tissues for cracking down on illegal water injection. A multiresidue method based on ultra performance liquid chromatography tandem mass spectrometry was developed for the determination of carbamazepine and its metabolites in animal matrix. In this method, samples were extracted by acidified acetonitrile, and purified by Oasis PRiME HLB SPE column, and then carbamazepine and its metabolites were analyzed by Acquity UPLC BEH C18 column (100 mm×2.1 mm, 1.7 μm) using methanol and 0.1% formic acid as the mobile phases. The signals were acquired through the MRM mode. All analytes could be well-separated by a gradient program during 8 minutes. The calibration curves of carbamazepine and its metabolites had a good linear in a concentration range of 1.0~50.0 μg/L with the correlation coefficient more than 0.999, and the limits of quantitation were all less than 1.0 μg/kg in animal matrix. The recoveries of 1.0, 10, 20 μg/kg fortified samples ranged from 72.3% to 106.8%, with RSDs of 1.43% to 10.3%. The new method provides a certain reference for the prevention of illicit drug addition and water injection in animal products.
