Analysis of the Composition and Function of Different Part Protein in Milk
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Abstract:
In this study, SDS-PAGE was used for separating of different component protein parts in milk, and mass spectrometry was used for identifying. It found that there were 201 kinds of protein in milk fat globule membrane, 96 kinds of protein in whey, and 43 kinds of protein in exosome and in these proteins, there were 27 kinds of the same expression protein. It found that the function of milk fat globule protein in the process of biological process was higher than whey protein and milk exosome protein, especially in biological regulation through the functional analysis of GO. In the molecular function, the main molecular function of different proteins in milk is the combination, and the function of milk fat globule proteins is the strongest. Exosome proteins involved in the molecule function of transport activity are greater than the milk fat globule membrane and whey proteins. Milk exosome involving in more cellular composition is the membrane, compared with whey and milk exosome, MFGM protein involved in cellular composition are more. KEGG pathway analysis shown that milk fat globule membrane, whey, milk exosome involved in metabolic pathways are not the same, metabolic pathways involved in the three parts was the peroxisome proliferator-activated receptor signaling pathway. Studying on milk protein can not only increase the utilization rate of milk, and the milk fat globule membrane, milk protein particles could provide a theoretical basis for producing dairy products as a raw material in the future.
