Determination of 4 Types of Polycyclic Aromatic Hydrocarbons Limited by EU Regulation in Edible Oils by Gas Chromatography Tandem Mass Spectrometry
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Abstract:
A new method was established based on molecular imprinting solid phase extraction and gas chromatography tandem mass spectrometry (GC-MS/MS) to determine 4 polycyclic aromatic hydrocarbons (PAH4) in edible oil limited by EU, including benz (a) anthrancene (BAA), chrysene (CHR), benzo (b) fluoranthene (BBF) and benzo (a) pyrene (BAP). . The edible vegetable oil sample was dissolved by n-hexane with CHR-d12 and BAP-d12 as internal standards, and then the sample solution was passed through a BAP molecularly imprinted cartridge connected a silica gel cartridge above. After discarding the silica gel cartridge, the molecularly imprinted cartridge was rinsed with n-hexane and eluted with dichloromethane. The eluent solution was concentrated with nitrogen flow, and the detection was performed using GC-MS/MS MRM mode. All analysis showed that 4 PAH had good linearity (R2>0.999) in the range of 1~50 μg/L. The limits of detection and quantitation of PAH4 were 0.21-0.49 μg/kg and 0.70-1.63 μg/kg, respectively. The recovery rate of spiked levels at 5, 10 and 20 g/kg was 81.06-111.30%, and the relative standard deviation was 0.86 -5.65%. -. The results showed that BAA, CHR, BBF and BAP were detected in all the 26 commercial edible vegetable oil samples. The content of BAP was 2.04 ~ 6.16 μg/kg, and the content of PAH4 was 9.36 - 31.38 μg/kg. According to the Chinese national food safety standard, the content of BAP was in the safety level, but the total amount of PAH4 should be controlled for improving the safety quality according to the European Union limit standard.
