Determination of Bezonic Acid in Zanthoxylum Using HPLC
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Abstract:
In this paper, the high performance liquid chromatography (HPLC) method was studied for benzoic acid content in zanthoxylum. Based on the complex chemical compositions of zanthoxylum, mixed anion solid phase extraction column was used to purify the sample in the pretreatment. The separation was performed at 230 nm with Waters sunfire C18 column and with phosphate buffer solution - methanol (95:5) as mobile phase. This method had good linear relationship in the range of 1.0 -1.0 mg/kg with R2 0.999 and LOD was 2.0 mg/kg, which was lower than the national standard GB 5009.28-2016 detection limit of 5.0 mg/kg. The recovery ratio at 2.0, 5.0, and 20.0 mg/kg levels was 73.1% -73.1%, while RSD was 2.8%- 7.4%. This method also provided reference for the determination of benzoic acid in other complex matrix. Then 40 samples of zanthoxylum in Sichuan province were analyzed, and benzoic acid was detected in 39 samples with the content of 2.08 - 12.20 mg/kg, and the average content was 6.02 mg/kg. This study preliminarily determined the background level of natural benzoic acid in Sichuan peppercorns, which provided an important basis for the formulation or revision of relevant standards in the food industry.
