Study on the protein Recovery from Potato Starch Processing Water by Ultrafiltration
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Abstract:
Potato starch processing water (PSPW) contains about 1.5% protein. The application of ultrafiltration in protein recovery from PSPW was analyzed in this study and the activity of protease inhititors was measured accurately by optimizing the experimental conditions. Under the experimental conditions, the results showed that: the protein concentration of the concentrated solution of ultrafiltration membrane packs of 10,000 MWCO and 30,000 MWCO increased to 4.35 and 3.90 times, respectively, and the protein recovery rates of the two ultrafiltration membrane packs were 67.61% and 62.98%, respectively, in the case of the concentration ratio of 5. SDS-PAGE results showed that there was no difference in protein composition between the two ultrafiltration membrane packs, both of which contained patatin protein and protease inhibitors, but the efficiency of 30000 MWCO was higher, because that the water and small molecules were easier to penetrate, so it was more suitable for the recovery of proteins of the PSPW. The trypsin inhibitor activities of ultrafiltration membrane packs of 10,000 MWCO and 30,000 MWCO were 124.38 and 95.25 mg/mg, respectively. Ultrafiltration is suitable for the recovery of natural active protein from potato starch processing juice.
