Optimization of Extraction Process of Total Flavonoids from Chimonanthus salicifolius S. Y. Hu. Leaves by Response Surface Methodology and Its Effect on Trypsin Activity
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Abstract:
Using Chimonanthus salicifolius S. Y. Hu. leaves as raw material, the total flavonoids of Chimonanthus salicifolius S. Y. Hu. leaves were extracted by ultrasonic-assisted method. The effects of ultrasonic time, ultrasonic power, ethanol volume fraction, solid-liquid ratio on the yield were investigated. On the basis of single factor tests, Box-Benhnken center combination experiment was used to carry out four factors and three levels experiment design. The yield of total flavonoids of Chimonanthus salicifolius S. Y. Hu. leaves was used as the response value for the response surface analysis, and the effect of the flavonoids purified from polyamide resin on the trypsin activity was explored. The optimal process conditions were as follows: ultrasonic time 30 min, ultrasonic power 57.59 W, ethanol concentration 52.03% and solid-liquid ratio 1: 23.85. Under these conditions, the yield of total flavonoids was 19.38% ± 0.12%. The relative error between the actual value and the predicted value was only 1.9%, and the coefficient of variation was only 0.6%. The extraction method also showed good repeatability. A second mathematical model of total flavonoids extracted from ethanol solution was established, which has a good predictive value for the extraction of the target product. The total flavonoids obtained were purified by polyamide resin and concentrated by elution with 30% ethanol. The half-inhibitory concentration value (IC50 value) of the purified flavonoids on trypsin was 1.36 mg/mL.
