Abstract:The enzymatic hydrolysis of schizophyllan by endo-β-1,3-glucanase produced by Schizophyllum commune fermentation was investigated to to achieve moderate molecular weight and better solubility of schizophyllan. The strain GIM5.43 with higher yield of polysaccharide and enzyme activity was selected from four stains(GIM5.42, GIM5.43, GIM5.44, Sc1),. The polysaccharide yield and the enzyme activity were 2.29 g/L and 0.28 U/mL, respectively. The addition time and dosage of optimum yeast extract and ammonium chloride for the growth of Schizophyllum commune, the yield of schizophyllan and the enzyme activity of β-1,3- glucanase were different. The batch feeding strategy was adopted to ensure the yield of schizophyllan. The initial concentration of yeast extract was 1 g/L and 0.10 g/L yeast extract was added on the 6th day. Under these conditions, the yield of schizophyllan was 4.16 g/L, which was improved by 61.24%, and the total enzyme activity was 0.34 U/mL, which was increased by 142.86%.
