Effects of Nitrogen Source on Schizophyllan and Beta -1, 3- glucanase Produced by Schizophyllum commune

doi:10.13982/j.mfst.1673-9078.2018.03.011

Archive > Volume 34 Issue 3 > 2018,34(3):77-82. DOI:10.13982/j.mfst.1673-9078.2018.03.011 Prev Next

Effects of Nitrogen Source on Schizophyllan and Beta -1, 3- glucanase Produced by Schizophyllum commune

HE Ling-ling
HE Ling-ling
(1.School of Food Science and Engineering, South China University of Technology, Guangzhou, 510640, China)
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WU Li-hua
WU Li-hua
(1.School of Food Science and Engineering, South China University of Technology, Guangzhou, 510640, China)
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ZHENG Bi-sheng
ZHENG Bi-sheng
(1.School of Food Science and Engineering, South China University of Technology, Guangzhou, 510640, China)
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HU Kang
HU Kang
(1.School of Food Science and Engineering, South China University of Technology, Guangzhou, 510640, China)
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YAN Sheng-fan
YAN Sheng-fan
(2.Guangdong Shidai Institute of Food and Life Sciences, Guangzhou 510670, China)
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JIANG Ze-gen
JIANG Ze-gen
(2.Guangdong Shidai Institute of Food and Life Sciences, Guangzhou 510670, China)
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Abstract:

The enzymatic hydrolysis of schizophyllan by endo-β-1,3-glucanase produced by Schizophyllum commune fermentation was investigated to to achieve moderate molecular weight and better solubility of schizophyllan. The strain GIM5.43 with higher yield of polysaccharide and enzyme activity was selected from four stains(GIM5.42, GIM5.43, GIM5.44, Sc1),. The polysaccharide yield and the enzyme activity were 2.29 g/L and 0.28 U/mL, respectively. The addition time and dosage of optimum yeast extract and ammonium chloride for the growth of Schizophyllum commune, the yield of schizophyllan and the enzyme activity of β-1,3- glucanase were different. The batch feeding strategy was adopted to ensure the yield of schizophyllan. The initial concentration of yeast extract was 1 g/L and 0.10 g/L yeast extract was added on the 6th day. Under these conditions, the yield of schizophyllan was 4.16 g/L, which was improved by 61.24%, and the total enzyme activity was 0.34 U/mL, which was increased by 142.86%.

Keywords:

schizophyllan ; endo- β-1,3- glucanase ; nitrogen source ; enzyme activity

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History

Manuscript received: September 07,2017

Online Published: April 03,2018

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