Correlation of the Antioxidant Activity and of Polysaccharides from Auricularia Auricula with its Molecular Weight and extracting agent Properties
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Abstract:
The Auricularia auricula polysaccharides were prepared from wild Auricularia auricula in this study and the molecular weight was fractionated by Labscale TFF System laboratory ultrafiltration system to investigate the effects of acid-base property of extraction solvent and molecular weight on the antioxidant activity of Auricularia auricula polysaccharides The yield, purity and antioxidant activity of the Auricularia auricula polysaccharides were determined and the correlation between molecular weight and antioxidant activity were analyzed by SPSS software. The highest yield of acidic non-decolorized polysaccharides (12.48%) and the highest purity of the alkaline decolorized polysaccharides (68.78%) were obtained, and the neutral decolorized Auricularia auricula polysaccharides had the best antioxidant activity in vitro .The antioxidant experiment of neutral non-decolorized Auricularia auricula polysaccharides with different molecular weights showed that the antioxidant activity of polysaccharides with the molecular weight of less than 30 ku was best, which was similar to that of Vc, followed by the polysaccharides with the molecular weight of 30 ku to 100 ku , and the worst were those with the molecular weight greater than 100 ku .There was a significant correlation between molecular weight and antioxidant activity(p<0.01). The results provided a theoretical basis for the further development of antioxidant products of Auricularia auricula polysaccharide.
