Preparation and Structure Analysis of Aurcularia auricular Polysaccharide from Jinggang Mountains
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Abstract:
The polysaccharides of Aurcularia auricular (AAP)sampled from Jinggang mountains, Jiangxi province, was extracted and structural identified. The optimal extraction method was established by comparing the content and yield of crude AAP by 4 different extraction methods, including ultrasound, cellulase, ultrasound synergistic cellulase, ultrasound synergistic cellulase and trypsin. The results showed that the content of AAP extracted by ultrasound synergistic cellulase method was the highest (85.93%), and the yield was 1.55%. Two kinds of A.auricular polysaccharides , named AAP3-20 and AAP3-60, were obtained when the volume fraction of isopropanol was 20% and 60%, respectively, after deproteinization, decoloration and alcohol precipitation. Monosaccharide analysis suggested that the monosaccharide composition of AAP3-20 was only glucose (77%) and the AAP3-60 was composed of mannose (51.91%), xylose (43.62%) and a small amount of glucose and galactose. Methylation analysis showed that the branching degree of AAP3-20 and AAP3-60 were 73.1% and 27.4%, respectively, and AAP-60 had an absorption peak at 669 cm-1, indicating that the polysaccharide might be in the form of β-pyran ring. This research could provide a theoretical basis for investigate the fine-strucure of AAP and the development of related products.
