Study on the Apple Polyphenols Release after Simulated Gastrointestinal Digestion and Its Antiproliferation Activity on HepG2 Cell
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Abstract:
The apple polyphenols were extracted by 80% acetone extraction. After simulated gastrointestinal digestion, , the polyphenols release of the extract and its antiproliferation activity on-HepG2 cell were determined. The results showed that the release contents of chlorogenic acid was the highest in the 80% acetone extracts from different apple varieties Crystal, Gala and Green, being of 30.72 ± 1.54, 22.37 ± 1.12 and 8.70 ± 0.44 mg/kg FW (Fresh weight, FW), respectively. After simulated gastric digestion, high amounts of protocatechuic acid, p-hydroxybenzoic acid, gallic acid, chlorogenic acid and other phenolic acids were released, while high contents of quercitrin, quercetin-3-glucoside, stearosine and other flavonols were released after simulated intestinal digestion. The cells survival rates were 26%, 42%, 44% and 23%, 28%, 28%, respectively, after HepG2 cells were treated with simulated gastric digestion and simulated intestine digestion of the apple extracts from Crystal, Gala and Green (concentrations of 297, 99, 255 mg/mL and 198, 154, 106 mg/mL, respectively) for 72 hours. This indicated that the simulated gastric digestion mainly promoted the release of phenolic acids, while the simulated intestinal digestion mainly promoted the release of flavonols. In a certain concentration range, apple extracts by simulated gastrointestinal digestion had strong antiproliferative activity against HepG2 cells.
