Protective Effect of Hazelnut Pentapeptide on Oxidative Stress Injury in HUVECs
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Abstract:
The antioxidant activity of a pentapeptide Ala-Trp-Asp-Pro-Glu (AWDPE) derivated from hazelnuts of Changbai Mountain was investigated and its protective effect on oxidative stress injury in HUVECs were also evaluateded . The results showed that when, the radical scavenging rates of AWDPE (1 mg/mL) on 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2‘-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) were 63.38 ± 2.44% and 69.38 ± 1.96%, respectively. Different concentrations of AWDPE had neither inhibitory nor promoting effects on the proliferation of HUVECs. HUVEC cell activity of AWDPE protection group was 1.51 times higher in than that of the angiotensin II (Ang II) injuried group (p < 0.01). The release rate of lactate dehydrogenase (LDH) in 100 μg/mL AWDPE protection group was 27.14 ± 1.16% , which was 0.62 times higher than that of the Ang II injuried group (p < 0.01). The methane dicarboxylic aldehyde (MDA) content in 500 μg/mL AWDPE protection group was 3.47 ± 0.28 nmol/mg, which was 0.73 times higher than that of Ang II injuried group. The content of catalase (CAT) in 100 μg/mL AWDPE protection group was 2.34 ± 0.11 U/mg, 1.39 times higher than that of the Ang II injuried group (p < 0.05). The total superoxide dismutase (T-SOD) content in 500 μg/mL AWDPE protection group was 23.55 ± 1.26 U/mg,2.48 times higher than that of the Ang II injuried group (p < 0.01). The glutathione peroxidase (GSH-PX) content was 33.9 ± 2.75 nmol/L in 500 μg/mL AWDPE protection group, which was 1.91 times higher than that of Ang II injuried group (p < 0.01 ). These results indicated that hazelnut pentapeptide AWDPE had significant protective effect against oxidative stress damage in HUVECs.
