Optimization of the Extraction Process of Tyrosinase Inhibitors from Gomphrena globosa by Response Surface Methodology
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
Inhibitory effect of Gomphrena globose (GG) crude extract on the tyrosinase activity was investigated and the extraction process of tyrosinase inhibitor was optimized by response surface methodology (RSM). Based on single-factor tests, the extraction temperature, extraction time, liquid-to-solid ratio and ethanol concentration were regarded as factors. A 4-factor, 3-level experiment design was performed using the Box-Benhnken center composite experiment. The response surface analysis was conducted by using tyrosinase inhibition rate as the response value, and the extraction process of the inhibitory component of tyrosinase activity was optimized.The results showed that the optimum extraction conditions by RSM were extraction temperature of 80℃, extracting time of 3 h, ratio of liquid to material of 60:1 (V/M) and the concentration of ethanol 50% (V/V). Under the conditions, the RSM model predicted that the inhibition rate of crude extract on tyrosinase was 54.94%, which was no significant difference compared with the actual measured value (53.86 ± 2.11)% (p > 0.05).
