The effects of iron and zinc biofortification on mineral elements and nutrient substance in soybean sprouts during germination were investigated. Soybean seeds were soaked and cultured with different concentrations of ferric sulfate (0~56 μg Fe/mL) , zinc sulfate (0~100 μg Zn/mL), and mixture solution, respectively. Four mineral elements were measured by Flame atomic absorption spectrometry(FAAS). The results showed that all the methods could increase the content of iron and zinc after soybean germination, and there was no significant difference in the effect of copper content (p > 0.05).. The results of this study showed that iron content in sprouted soybean seeds increased significantly (p < 0.05) as the internal Fe concentrations increased by 450.59% (FeSO4+ZnSO4, 16.8~33.6 μg Fe/mL, 30~60 μg Zn/mL) and 203.74%(FeSO4, 16.8~33.6 μg Fe/mL), respectively. The addition of ZnSO4 during iron biofortification could promote the iron accumulation of soybean sprouts, while FeSO4 had a negative impact on zinc accumulation of soybean sprouts during zinc biofortification. Ferritin content increased during culturing in doses of 16.8~33.6 μg/mL of FeSO4 with 30~60 μg/mL of ZnSO4. The antioxidant activity of soybean was the strongest during culturing in doses of 39.2 ug Fe/mL FeSO4 and 70 ug Zn/mL ZnSO4. In conclusion, we demonstrated that iron and zinc fortification during the germination process had a positive effect on iron and ferritin accumulation of soybean sprouts.
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