Rapid Determination of Wall-broken Rate of Beans Cells Based on Conductivity
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Abstract:
A method for rapid and accurate determination of wall-broken rate of beans cells by conductivity was established in this study. The relationship between water-soluble proteins of four kinds of beans and conductivity was investigated by measuring the protein content and conductivity of four kinds of beans with different degrees of treatment, and the effects of soymilk dosage, extravasation time and measure temperature on the wall-broken rate were also studied. The wall-broken rate of four common beans under the soymilk program of high speed blender was determined by using the established evaluation method. Results suggested that the correlation between the concentration of water-soluble proteins in the four common beans and the conductivity was significant (R2≥0.8995), and the soymilk dosage and extravasation time had no significant influence on the determination of wall-broken rate (p>0.05). The effect of measure temperature on wall-broken rate was very significant (p<0.01), and the wall-broken rate was relatively stable at 25 oC. The wall-broken rate of four common beans reached about 90% by using the new high speed blender. Consequently, it was feasible to quickly evaluate the wall-broken rate of beans cells by conductivity.
