Antimicrobial Resistance Characteristics and Epidemiology of Class Ⅰ Integron of Campylobacter Isolated from Chicken Meat in Jiangsu Province
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Abstract:
Campylobacter isolated from chicken meat in Jiangsu province during 2016-2017 were used as the study subject to investigate the antimicrobial resistance characteristics of campylobacter in chicken meat. K-B method was used to examine for antimicrobial susceptibility to 21 antibiotics in 9 categories, the class Ⅰ integrase gene (int1) and 3’-CS region, and the gene cassette of class Ⅰ integron were amplified by PCR . The results revealed that the resistance of campylobacter isolated strains to ciprofloxacin, cefotaxime, loxacin, kanamycin and ampicillin were 83.16%, 80.0%, 68.0%, 65.0% and 64.2%, respectively. Int1 was detected in 47.3% (45/95) of the Campylobacter isolates and 10 stains contained 3’-CS region, of which 5 strains were positive for gene cassette detected by PCR. The sequencing analysis demonstrated that the addA1 gene cassette encoded resistance to streptomycin was identified. The results suggested that the multiple resistance of the Campylobacter isolated from chicken meat were serious. The mechanism of multidrug resistance mediated by integrons might not represent a significant mechanism for the dissemination of antimicrobial resistance determinants in Campylobacter in Jiangsu province. However, the drug resistance gene in class I integrons could be transmitted to human through different bacteria via the food-chains. The multiple resistance and prevalence of class I integrons in chicken meat should be concerned strictly.
