Protective Effect of Acanthopanax Senticosus Extract on the Ultraviolet Radiation Injury of Human Umbilical Vein Endothelial Cells

Abstract:

The effect of Acanthopanax senticosus extract on the ultraviolet radiation injury of human umbilical vein endothelial cells (HUVEC) was investigated. The HUVEC cells entering the logarithmic growth phase were divided into blank control group, radiation group, drug prevention group (low, middle and high dose) and drug repair group (low, middle and high dose). Each group received the same radiation, and the drug prevention group was hatched for 12 h by adding different concentrations of Acanthopanax senticosus extract. The cell viability was measured by MTT assay and the content of malondialdehyde (MDA) was detected by TBA method. The level of superoxide dismutase (SOD) was detected by WST method. The apoptosis was detected by DNA-Ladder. The result showed that after radiation, the cell viability in the drug prevention group and the drug repair group were both increased in a concentration-dependent manner. Compared with the blank control group, the content of MDA in radiation group increased and the level of SOD decreased. Compared with the radiation group, the content of MDA decreased and the level of SOD increased in the experimental group. DNA-Ladder could obtain obvious DNA apoptotic bands. Acanthopanax senticosus extract could effectively prevent and repair radiation-injured HUVEC cells, and the preventive effect of the drug prevention group of the above-mentioned test was superior to the drug repair group.