Physicochemical Properties of Polysaccharides from HoneyComb and their Immunomodulatory and Anti-oxidative Effects on Immunosuppressed Mice
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Abstract:
In this study, physicochemical properties, acute toxicity, immunomodulatory and anti-oxidative effects of polysaccharides isolated from honeycomb on the immunosuppressed mice were analyzed. Physicochemical properties of polysaccharides isolated were investigated by the high performance liquid chromatography and spectrophotometry. The results showed that honeycomb polysaccharides contained 75.77% total sugar, 13.83% protein, 0.19% uronic acid, 4.20% reducing sugar, and they did not contain polyphenols. The composition of honeycomb polysaccharides mainly includes arabinose, glucose, galactose and so on. The oral acute toxicity test revealed that the oral tolerance dose of honeycombe polysaccharides for both male and female mice was larger than 10000 mg/kg, which belonged to the actual non-toxic level. Immunosuppressive mice model was established, and immunosuppressed mice were administrated at different doses of honeycomb polysaccharides. The results showed that compared with the model group, all the different doses of polysaccharides could significantly increase the content of hemolysin and the activity of superoxide dismutase (SOD) and catalase (CAT), and also significantly decrease the level of malondialdehyde (MDA) in serum. The medium-dose group showed significant differences compared to the model group (p < 0.01). In this dosage, the OD value, the activity of SOD and CAT in serum were increased to 0.09, 125.07 U/mgprot and 13.85 U/ml respectively. In addition, the content of MDA decreased to 4.89 nmol/ml. This result indicated that the honeycomb polysaccharides could effectively antagonize cyclophosphamide-induced immune suppression and enhance antioxidant function in immunosuppressed mice, had good development potential in the field of medicine and food.
