Ginsenosides of Ginseng Enzymatic Hydrolysate and Their Effects on Myeloid Derived Suppressed Cell
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Abstract:
A quantitative method for simultaneous determination of 12 ginsenosides in ginseng enzymatic hydrolysate was established by using high performance liquid chromatography (HPLC). The differences of main ginsenosides between ginseng extracts and ginseng enzymatic hydrolysates were investigated., and the effects of ginseng extract on myeloid derived suppressed cell (MDSC) before and after enzymolysis was studied. The differences of total saponin content between ginseng extracts and ginseng enzymatic hydrolysates were not obvious, and the content of 12 ginsenosides and rare ginsenosides (Rh1, F1, F2, Rg3, CK and Rh2) in hydrolysates were significantly higher than those in ginseng extracts. After enzymatic hydrolysis, the content of rare ginsenosides in ginseng enzymatic hydrolysates was increased by 4.48 times, especially a large number of rare ginsenosides of F2, and a small amount of rare ginsenosides of F1, CK and Rh2 were produced. Ginseng extracts and ginseng enzymatic hydrolyzates could significantly inhibite the proliferation of MSC2 cells and reduce the proportion of MDSC cells in the spleen of colon cancer-bearing mice, and the effects of hydrolyzates were better than that of ginseng extract. Compared to ginseng extracts, the inhibition rate of ginseng enzymatic hydrolysates on MSC2 cells was increased by 30.00% and the proportion of MDSC cells was decreased by 40.50%. Consequently, the ginseng enzymatic hydrolysates were rich in rare ginsenosides and had a higher biological activity, which could effectively improve the tumor microenvironment and then enhance the anti-tumor ability.
