The Effect of Lilium lancifolium Anthocyanin on the Improvement of Liver Fibrosis Induced by CCl4 in Rats
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Abstract:
In order to explore the prevention, improvement and possible mechanism of alcohol extract anthocyanin from Lilium lancifolium on the liver fibrosis induced by CCl4 in rats, thirty-two rats were randomly divided into control group (Sham), liver fibrosis model group (M), low dose lancifolium anthocyanin group (LFF 0.5 g/mL) and high dose lancifolium anthocyanin group (LFF 1.0 g/mL). Rats in M group and LFF group were subcutaneously injected with 50% CCl4 on the back, twice a week for 8 weeks. The rats in M group and LFF group were subcutaneously injected with 50% CCl4 on the back twice a week for 8 weeks. The rats in LFF group were given different doses of alcohol extract anthocyanin from Lilium lancifolium. Sham group and M group were given the same volume of physiological saline once a day. After eight weeks, the animals were sacrificed and serum was collected for detecting alanine amiotransferase (ALT) and aspartate aminotransferase (AST). The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-px) and the content of malondialdehyde (MDA) in the liver homogenate were measured. Western blot was employed to examine the expression of transforming growth factor-β 1 (TGF-β1) and alpha-smooth muscle actin (α-SMA) in the liver tissue. The results showed that compared with M group, Lilium lancifolium anthocyanins could obviously reduce the ALT and AST levels in rat serum, and significantly increase the activities of SOD, CAT and GSH-Px in liver tissue, decrease the lipid peroxideMDA, and then down-regulate the expression of liver fibrosis related materials such as TGF-β1 and α- SMA. Lilium lancifolium anthocyanins had good antioxidant activity, which could significantly reverse the liver fibrosis induced by CCl4. The mechanism might be related to resist the damage of lipid peroxide and reduce the proliferation of extracellular fibrous tissue.
