Development of a Multi-Residue Colloidal Gold Detection Card for New Type Lean Meat Powder
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Abstract:
A new method of multi-residue colloidal gold detection card for detecting lean meat powder was estiblished and could be used for quantitative detection of a large number of samples. Applicated, Be used Colloidal gold immunochromatography assay and sodium citrate reduction method were used to mark the new type lean meat powder antibody, which was then sprayed onto a glass fiber. The new type lean meat powder was coupled with OVA and goat anti-mouse IgG on nitrocellulose membrane, and the sample pad, colloidal gold pad, nitrocellulose membrane and absorbent paper were assembled and cut into multi-mesidue colloidal gold detection cards for new type lean meat powder. The gradient color results obtained by investigating sensitivity, false positiveand false negative, showed that the sensitivities of the detection card were as follows: clenbuterol, 3 ng/mL, ractopamine, 5 ng/mL, salbutamol, 3 ng/mL, sima Tero, 5 ng/mL, bambuterol, 5 ng/mL, tulobuterol, 5 ng/mL, terbutaline, 8 ng/mL, clorprenaline, 5 ng/mL, mabuterol 5 ng/mL, brombuterol 8 ng/mL. The detection time was 3 min, and the optimal urine volume was 70~90 mL. In addition, the intra-and inter-reproducibility was 100%, and the false positive rate and false negative rates were both 0. The joint inspection technology, which could be used to supervise the production process and screen large quantities of samples, could realize rapid detection, improve the detection speed and accuracy and reduce the cost.
