Abstract:The determination of deoxynivalenol in wheat flour by high performance liquid chromatography was studied in this paper. The detection of deoxynivalenol in wheat flour was very important for the storage and quality control of wheat flour, and the chromatographic conditions of immunoaffinity-high performance liquid chromatography, specified by national standard-GB 5009-2016, had a risk of false positives for the determination of deoxynivalenol in wheat flour. Conditions of liquid chromatography ,optimized based on national standard, were as follows: mobile phase, methanol-water (25:75, V/V); detection wavelength, 240 nm; scanning range,190 nm~400nm.In addition, the qualitative analysis was also studied using full wavelength scanning by diode array detector. The results showed that the recovery was 91.7% ~ 97.2% and the detection limit was 20 μg/kg, the linear correlation coefficient was 0.99989 in the concentration range of 50 ng/mL ~ 5000 ng/mLwith the precision of 0.83% ~ 3.21%. Consequently, the optimized method could not only avoid the risk of false positives by qualitative analysis, but maintain a high recovery rate and precision, wide linear range and low detection limit, which could be better and more accurately used for the detection of deoxynivalenol in wheat flour.
