Quantitative Analysis of Mutton and Pork in Meat Products by Micro Digital PCR
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Abstract:
In order to realize the quantitative research of mutton and pork in meat products, a quantitative method based on the droplet digital polymerase chain reaction (ddPCR) was used for quantitative detection of meat products in this study. It was better to identify the adulteration of human factors or inadvertent bringing into during the process by designing single primers on housekeeping genes. The results of ddPCR showed that the relationships between the DNA concentration and DNA copy number(C) were close to be linear in a certain rage of meat weight and DNA concentration. This method was validated by artificial adulteration and commercial sample detection. The DNA concentration was utilized as an intermediate value to establish the formulae about the relationship between the original meat weight and DNA copy number: Movine=0.12C-10.6, Mpork=0.07C+4. By examining the mixed meat samples of known adulteration composition and commercially available samples, a more accurate method for detecting the adulteration meat was established. It shows that there is a certain adulteration in the market, and the droplet digital polymerase chain reaction(ddPCR) established in this paper has great potential in adulteration detection of mutton and pork and their products, which provides a scientific basis for the market supervision of adulterated meat products at present.
