Simultaneous Determination of Five Sterols in Infant Formula Rice Flour by Solid Phase Extraction-Isotope Dilution-Gas Chromatography-Mass Spectrometry
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Abstract:
A method for simultaneous determination of 5 sterols in infant formula rice flour by solid phase extraction-isotope dilution- gas chromatography-mass spectrometry was established in this study. Samples were separated and extracted on a SPE column without saponification and derivatization and purified by adding deuterium isotope internal standard withsolid phase extraction. Then target sterols were separated on a HP-5MS (30 m×0.25 mm×0.25 m) column, detected by a selected ion monitoring (SIM) mode and quantified by internal standard method, which could achieve the accurate characterization and quantification of sterols. The results showed that the fivesterols had good linearity in their respective linear ranges with the correlation coefficients greater than 0.9995, and the detectionlimits (S/N = 3) were all 50 g/kg for. In addition, the recoveries were 91.9%~107% and the relative standard deviations (RSDs, n = 6) were in the range of 3.1~4.6%. The proposed method had the advantages of simple pretreatment, high sensitivity, good reproducibility and accuracy, as well as scientificity and operability, which was suitable for the simultaneous determination of 5 sterols in infant formula rice flour.
