Extraction and Purification of Procyanidins from Black Bean Seed Coats
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Abstract:
In this study, extraction and purification methods of procyanidins from black bean seed coats was investigated. The procyanidins (PC) were extracted by ultrasonic assisted extraction and the crude extracts were purified by AB-8 macroporous resin adsorption method. The purified products were analyzed by infrared spectrum. The results of single factor experiments showed that the extraction method was more relatively effective when the concentration of ethanol was 60%, the extraction time was 50 mins, the solid-liquid ratio was 1:20 (m/V) and extraction temperature was 50℃. Whereafter, the results of orthogonal test showed that the optimal extraction conditions were as follows: the ethanol concentration of 60%, the extraction time of 50 mins, the solid-liquid ratio of 1:20 (m/V), and the extraction temperature of 60℃. The optimal dynamic adsorption and analytical conditions of the purification of AB-8 macroporous resin were as follows: 0.6 mg/mL procyanidins extract, the flow rate of 1 mL/min, the adsorption equilibrium time of 4 h, 60% ethanol solution as the eluent, elution flow rate of 1.5 mL/min, the elution balance time of 3 h. Qualitative analysis by infrared spectroscopy showed that the above conditions for the purification of proanthocyanidins were relatively effective.
