Preparation Collagen Peptides from Antlers of Red Deer (Cervus elaphus) with the Activity of Promoting Proliferation of Human Skin Fibroblasts by Bi-enzymatic Hydrolysis
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Abstract:
As the main component of the extracellular matrix, collagen was closely related to the proliferation and differentiation of the cells. In order to explore the suitable preparation conditions of the collagen peptides from red deer (Cervus elaphus) antler with the function of promoting the proliferation of the skin fibroblasts, the antler was used as the raw material and hydrolyzed by alcalase and trypsin . The degree of hydrolysis (DH), peptides yield and the proliferation rate of normal human dermal fibroblasts (NHDF) were used as the target to perform the single factor experiments and orthogonal experiments and determine the appropriate process conditions. The results showed that the optimal preparation conditions were as follows: alcalase was used for enzymolysis under the conditions of substrate concentration of 1%, enzyme substrate ratio of 2%, pH 9.0 and temperature of 60 ℃, and then trypsin was used for enzymolysis under the conditions of substrate concentration of 1%, enzyme substrate ratio of 6%, pH 8.0 and temperature of 42 ℃. The final hydrolysis degree of the hydrolyzate, the yield of peptides and proliferation rate of cells were 21.7%, 86% and 22.75%, respectively. Collagen peptide with promoting the proliferation rate of NHDF could be obtained from red deer antler by alcalase-trypsin bi-enzyme hydrolysis.
