Effects of EGCG on the Lipid Metabolism in Obese HFA Mice Induced by High-Fat-Diet
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Abstract:
The lipid-lowering effects of (-)-epigallocatechin-3-gallate (EGCG) on obese human flora-associate (HFA) mice induced by high fat diet were investigated in this study. The aseptic Kunming (KM) mice were inoculated with healthy human excrement suspension to construct HFA model and randomly divided into two groups: high-fat group (40) and normal group (10) were fed with high-fat diet and normal diet, respectively. After 8 weeks, the mice were sacrificed and the body weight, liver weight, epididymal fat pad weigh, blood glucose, blood lipid and hematoxylin and eosin (HE) staining of liver and epididymal fat pad were measured. The obese mice were randomly divided into three groups: the control group, 0.2% EGCG low-dose group and 0.8% EGCG high-dose group, and fed with normal diet After 8 weeks, the mice were sacrificed and several indices mentioned above were assayed. The results showed that the epidihymal fat pad weight,total cholesterol (Tch) and low density lipoprotein content (LDL-C) in high dose group were significantly decreased by 48.15%, 28.64% and 43.16%, respectively, as compared to the normal group. Furthermore, EGCG treatment also normalized the cellular morphology of the epidihymal fat pad, and alleviated hepatic damage in a dose-dependent manner. These results suggested that EGCG had certain effects on lipid-decreasing and liver injury repair activity in obese HFA mice.
