Detection of Alicyclobacillus spp. Contamination in 6 Commercial Beverages by Electronic Nose
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Abstract:
Alicyclobacillus has the character of high tolerance to acid and heat and the ability of producing spore, which can germinate and propagate in the fruit juice and then make the products smell bad and cause corruption. In this study, the isolated strain XC-6 and standard strain DSM 3922 obtained from the German bacterial collection center were inoculated equally into 6 kinds of fruit juices, respectively. FOX 4000 electronic nose combined with principal component analysis was used for the analysis of inoculated samples and normal samples. The results showed that the electronic nose could identify the contaminated samples quickly and effectively from 6 kinds of fruit juices and’ the cumulative variance contribution rates of the first principal component were more than 95%. GC-MS analysis showed that the prints of volatile compounds between inoculated samples and normal samples were different. The difference of some typical substances content could be used to identify contaminated samples and normal samples by electronic nose. Adjacent ethoxy phenol and guaiacol, the characteristic metabolites of Alicyclobacillus, were detected in the peach beverage and fruit milk mixed drinks, of which the content was significantly higher than the normal samples. The experiments showed that the electronic nose could identify the contaminated samples accurately according to the difference of the juice samples smell maps, which could be used as a testing tool for early detection of Alicyclobacillus pollution.
