Protein Recovery and DPPH Radical Scavenging Activity of Enzymolyzed Products of Moringa Oleifera Seed
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Abstract:
The enzymolyzed products of Moringa Oleifera seed were prepared by aqueous enzymatic extraction. The protein recovery and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of the enzymolyzed products were selected as indexes to optimize the enzymatic hydrolysis conditions . Single factor experiment showed that the enzymolyzed products obtained by Alcalas 2.4L and Ns 37071 had the highest protein recovery and DPPH radical scavenging activity. The protein recovery were 45.65% and 47.65%, respectively, and the DPPH free radical scavenging activity were 99.88 and 93.89 μmol Trolox equiv/100 g, respectively. Further, the optimal enzymolysis conditions confirmed by response surface methodology were as follows: the total amount of enzyme 2.60%, Ns37071 accounted for 46% of the total amount of enzyme, and enzyme time 24 hours. The protein recovery was 63.69±2.94%, and DPPH radical scavenging activity was 139.38±0.96 μmol Trolox equiv/100 g. The study in this paper showed that the aqueous enzymatic extraction could efficiently hydrolyze Moringa oleifera seed, and enzymolyzed products had better protein recovery and DPPH radical scavenging activity.
