Structural Identification of Phenolic Constituents from Phyllanthus emblica L. and Their Inhibitory Activity on α-glucosidase
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Abstract:
The phenolic constituents in the hypoglycemic active fraction of the methanol extract of Phyllanthus emblica L and their inhibitory activity on α-glucosidase were studied. Using methanol soaking extract Phyllanthus emblica L, the methanol extract (PE) was chromatographed on a D101 macroporous resin column to obtain three fractions (PEA-PEC). Hypoglycemic activity test showed that 30% ethanol elution part (PEA) was the active fraction. PEA was further isolated and purified by column chromatography of Sephadex LH-20, ODS and semi-preparative HPLC columns. The structures of the isolated compounds were identified according to the physicochemical properties, 1H-NMR, 13C-NMR and high resolution mass spectral analyses as well as relevant references. The compounds were tested for their in vitro inhibitory activity on α-glucosidase. Seven phenolic compounds were isolated from PEA fraction and identified as gallic acid (1), methyl gallate (2), 1-O-galloyl-β-D-glucose (3), 1,6-di-O-galloyl-β-D-glucose (4), corilagin (5), mucic acid-1,4-Lactone-2-O-gallate (6), mucic acid-2-O-gallate (7). All compounds except compound 2 showed good inhibitory activity on α-glucosidase. Gallic acid derivatives were the active constituents that P. emblica L inhibited the activity of α-glucosidase.
