Study on the Correlation between Contents of Polyphenols, Flavonoids, Polysaccharides in Pinus koraiensis Seed Putamina and Their Antioxidant Activities
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Abstract:
In order to investigate the correlation between contents of major active ingredients in Pinus koraiensis seed putamina and its antioxidant activity, Yichun Pinus koraiensis seed putamina was used as raw material in this study to extract polyphenols, flavonoids and polysaccharides by different ethanol concentrations. The correlation between active ingredients in Pinus koraiensis seed putamina and their antioxidant activities were analyzed by SPSS statistical analysis software. The results showed that when the concentration of ethanol was 40%, the highest yields of polyphenols, flavonoids and polysaccharides in Pinus koraiensis seed putamina were 4.05 mg/g, 1.7 mg/g and 7.05 mg/g, respectively. Antioxidant activity test showed that antioxidant activity of polyphenols from Pinus koraiensis seed putamina was significantly higher than that of flavonoids and polysaccharides, which was comparable to that of Vc. The IC50 (half clearance concentration) values of polyphenols from Pinus koraiensis seed putamina on the 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical (·OH) and the total reducing power were 0.20±0.05 μg/mL, 13.04±0.04 μg/mL and 40.15±0.06 μg/mL, respectively. The correlation analysis showed that the correlation between the polyphenols from Pinus koraiensis seed putamina and the antioxidant activity was the highest, and the correlations between the polyphenols from Pinus koraiensis seed putamina and DPPH,·OH as well as the total reducing power were 0.908, 0.989 and 0.989 (p<0.01), respectively. It could be seen that polyphenols from Pinus koraiensis seed putamina were the main antioxidant components. The results of this study provided a scientific basis for the comprehensive utilization of the by-products of the Yichun Pinus koraiensis seed putamina and the development of natural antioxidants.
