Effects of 6-shogaol on H2O2-Induced Oxidative Damage in NCM460 and HCT116
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Abstract:
In this study, the model of oxidative damage in colorectal cancer cells was established. After the stimulation of hydrogen peroxide (H2O2), the different effects and possible molecular mechanisms of 6-shogoal on H2O2-induced oxidative damage in human intestinal epithelial cells (NCM460) and in situ colon cancer cells (HCT116) cells in vitro were investigated. Inverted microscope was used to observe the changes of cell morphology in HCT116 and NCM460 induced by H2O2 with different concentrations of 6-shogoal. CCK-8 (Cell Counting Kit-8) method was used to screen the concentration interval of 6-shogaol and the cell survival rate was determined. Annexin and V-FITC/PI cytometry were used to detect apoptosis of different groups. The expressions of related apoptotic proteins (Caspase-3, poly (ADP- ribose) polymerase-1 (PARP-1), MCC1, A2F, B-cell lymphoma-2 (Bcl-2)) were detected by Western-blot. As compared with the model group, 6-shogaol could reduce the expressions of caspase-3, PARP-1, MCC-1, A2F and promote the expression of Bcl-2 (p < 0.05) in NCM460 induced by H2O2, indicating that 6-shogaol had anti-oxidant and pro-proliferative effects (p<0.05). However, 6-shogoal could promote the expression of caspase3, PARP-1, MCC1 and A2F, and inhibit the expression of Bcl-2 (p<0.05) in HCT116 induced by H2O2, indicating that 6-shogaol could enhance the oxidative damage of HCT116 induced by H2O2 and inhibit cell proliferation (p<0.05). It could be concluded that 6-shogaol had different opposite effects in NCM460 and HCT116 induced by H2O2, which might provide guidance for further studies on specific mechanisms or pathways of 6-shogoal against colorectal cancer.
