Preparation and Adsorption Properties of Enrofloxacin MolecularImprinted Polymer Film
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Abstract:
The enrofloxacin molecular imprinted polymer film was directly synthesized on the surface of polystyrene microtiter plate by surface imprinting technique using the enrofloxacin (ENRO) as template, methacrylic acid (MAA) as functional monomer and ethyleneglycol dimethacrylate (EDMA) as cross-linker. The enrofloxacin molecular imprinted polymer film was characterizedby FT-IR, scanning electron microscope, adsorption-equilibrium experiments, scatchard analysis and adsorption kinetic experiments. The results showed that the imprinted film exhibited good imprinting effect, high specific adsorption ability and fast mass transfer rate. Scatchard analysis indicated that the imprinted film contained two types of binding sites with specific binding properties. The dissociation constant (Kd) and maximum binding capacity (Qmax) of the two linear curves were 19.49μg/mL, 12.98 μg/mL for the higher affinity sites and 277.78 μg/mL, 98.14 μg/mL for the lower affinity sites, respectively, and the heterogeneous of MIP could be ignored in MIAs. The enrofloxacin- specific polymer film synthesized by the proposed method could be used as a biomimetic antibody to determine the enrofloxacin residues in food by competitive immunosorbent assay.
