Screening the Saccharomyces Cerevisiae with High Temperature Toleranceby ARTP Mutagenesis and Genome Shuffling
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Abstract:
Saccharomyces cerevisiae has the advantages of fast fermentation speed, short fermentation cycle, low viscosity of fermentation raw materials and low production cost during the high temperature fermentation in industrial production, which is more suitable for simultaneous saccharification fermentation technique. Therefore, S. cerevisiae strain with high temperature tolerance is of great significance in the industrial application. In this study, AY12 was used as the starting strain, which was isolated from industrial strains. The strain was mutated by atmospheric and screened at 37 ℃. Then, 150 strains with good colony growth were obtained. By the way, the stains were carried out to achieve genome shuffling by sporulation and hybridization, and 137 strains with good growth performance were obtained by re-screening at 37℃. After fermentation in the corn hydrolysate at 35 ℃, 14 strains were obtained with good fermentation performance. The strains were rescreened by the simultaneous saccharification fermentation at 35 ℃, and the ethanol yield and residual sugar content of each strain were determined. Finally, 7 strains were screened with good tolerance to high temperature fermentation. L-38 was screened as the best fermentation performance strain after the simultaneous saccharification fermentation. Compared with the starting strain AY12, the ethanol yield of L-38 was 16.20% (V/V), which increased by 8.00%., and the residual sugar content was 35.50 g/L, which was 32.38% lower than that by AY12.
